Estudio de la prevalencia de Mycoplasma genitalium en una población de mujeres en Córdoba.

MARTIN MASNER; KIGUEN; LUJEA, NOELIA; GANZER; LARCHER; CUFFINI, CECILIA; KUNDA, PATRICIA

Salta

Congreso; LV Annual SAIB Meeting and XIV PABMB Congress; 2020

Mycoplasmosis caused by Mycoplasma genitalium (MG) is considered in recent years as one of the emerging sexually transmitted diseases. The infection has been associated with vaginitis, spontaneous abortions, urethritis, etc. Little is known about the existing genotypes and the prevalence in our Province.Even less is known of the relationship between this bacterium and infertility and cancer. MG is difficult to grow because it needs special growing conditions. In addition, the growth of mycoplasma is slow, so the diagnosis based on the plate culture technique can take three or more weeks. The PCR technique, on the other hand, is fast, and highly sensitive and specific. Given the clinical and sanitary importance of MG and the difficulty of diagnosing it, we set out the PCR-based methodology for specific detection of M. genitalium and study the prevalence of mycoplasmosis in women in Córdoba.Genomic DNA extracted from vaginal exudates of gynecological consultation patients from the Private Hospital and the National Hospital of Clinics, were used for the study. The project has the approvalfrom the HPUC CIEIS and it was approved by the Research and Teaching Committee from the HPUC for the retrospective analysis of samples from the National Hospital of Clinics. The presence of MG and Ureaplasma was determined by PCR using different primers directed against the DNA of the 16S ribosmal subunit of the bacteria.The results showed that MG is present in 4% of the patients analyzed while Ureaplasma has an incidence close to 50%.This is the first report of the presence of MG in these health centers, and the first in Córdoba based on molecular methods. We believe that being able to have a diagnostic method of high sensitivity, specificity and speed will result in a better treatment and patient response.