INVESTIGADORES
KUNDA Patricia Elena
congresos y reuniones científicas
Título:
Moesin controls cortical rigidity, cell rounding and spindle assembly during mitosis
Autor/es:
KUNDA, PATRICIA
Lugar:
Dresden
Reunión:
Workshop; Blebs and Cells Cortex Mechanics in Cells Movements Conference; 2008
Resumen:
<!--
/* Font Definitions */
@font-face
{font-family:Arial;
panose-1:2 11 6 4 2 2 2 2 2 4;
mso-font-charset:0;
mso-generic-font-family:auto;
mso-font-pitch:variable;
mso-font-signature:-536859905 -1073711037 9 0 511 0;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;
mso-font-charset:1;
mso-generic-font-family:roman;
mso-font-format:other;
mso-font-pitch:variable;
mso-font-signature:0 0 0 0 0 0;}
@font-face
{font-family:"MS Mincho";
panose-1:0 0 0 0 0 0 0 0 0 0;
mso-font-alt:"ＭＳ 明朝";
mso-font-charset:128;
mso-generic-font-family:roman;
mso-font-format:other;
mso-font-pitch:fixed;
mso-font-signature:1 134676480 16 0 131072 0;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{mso-style-unhide:no;
mso-style-qformat:yes;
mso-style-parent:"";
margin:0cm;
margin-bottom:.0001pt;
mso-pagination:widow-orphan;
font-size:12.0pt;
font-family:"Times New Roman";
mso-fareast-font-family:"MS Mincho";
mso-ansi-language:EN-US;
mso-fareast-language:JA;}
.MsoChpDefault
{mso-style-type:export-only;
mso-default-props:yes;
font-size:10.0pt;
mso-ansi-font-size:10.0pt;
mso-bidi-font-size:10.0pt;}
@page WordSection1
{size:612.0pt 792.0pt;
margin:72.0pt 90.0pt 72.0pt 90.0pt;
mso-header-margin:36.0pt;
mso-footer-margin:36.0pt;
mso-paper-source:0;}
div.WordSection1
{page:WordSection1;}
-->
During mitosis, cells undergo a sequence of morphological
changes that include retraction of the cell margin, rounding, pole elongation,
invagination of the equatorial zone, cleavage, separation and the respreading
of the two daughter cells. Failure in one of the steps could leads to failure
in cell division and chromosome segregation, a cause for several diseases such
a cancer.
Using a combination of RNAi, cell biology and biochemical
techniques to study changes in the structure and dynamics of the actin cytoskeleton
in cultured Drosophila cell
lines, we identified Moesin as a key regulator of mitotic progression through
its effects on organization of the actin cell cortex, spindle morphogenesis and
chromosome alignment.
Additionally, since tumours
are known to have defects in cortical rigidity and genomic instability, we use
a graded series of cancer cell lines, from benign to malignant and we applied
similar experimental approaches used in Drosophila as above. We determined levels of mitotic active ERM to
correlate with cortical defects in human cancer cell lines By using imaging and AFM, we showed a relationships between cortical
tension, active ERM and tumorgnesis.
Furthermore, we analyzed aneuploidy linking the cortical defects with
the development of malignancy.
