Assessment of Shiga toxin producing Escherichia coli O157:H7 pathogenicity in the presence of short chain fatty acids and lactate.

ROMINA JIMENA FERNÁNDEZ BRANDO; ALAN MAURO BERNAL; FERNANDO NICOLÁS SOSA; MARÍA VICTORIA RAMOS; MARINA SANDRA PALERMO

Buenos Aires

Conferencia; Reunión Anual de Sociedades de Biociencias; 2021

SAI/SAIC

Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 is afoodborne pathogen, which can lead to the life-threatening Hemolytic Uremic Syndrome (HUS). There is no treatment available in orderto reduce HUS outcome up to date. Short chain fatty acids (SCFAs),including acetate (A) and butyrate (B), are produced by the intestinalmicrobiota. Lactate (L) is the main metabolite of many fermentedproducts. A, B and L can protect mucosa exposed to inflammatoryinsults. The aim of this work was to evaluate the effects of A, B andL on modulating STEC pathogenicity. We evaluated the inhibition ofSTEC growth after 3h culture, EspB production by SDS-PAGE andbacterial adherence to Caco-2 cells. We found that 50 mM of A andB, and 100 mM of the three compounds inhibited bacterial growthsignificantly compared to control: 0;1;10;50 and 100mM (medianCFU(IQR) x109) A=2.2(1.8-2.7);2.3(2.0-2.6);2.2(1.8-2.4);1.4(1.2-2.0);1.1(0.5-2.0);B=2.1(1.9-2.3);1.9(1.8-2.2);1.6(1.5-2.0);1.3(1.0-1.4);0.5(0.4-1.0);L= 2.1(1.7-2.5);2.3(1.6-2.6);1.7(1.5-2.1);1.6(1.5-1.9);1.1(1.0-1.5); p