Effect of intramyocardial administration of baculovirus encoding the transcription factor tbx20 in sheep with experimental acute myocardial infarction

BAUZÁ, MARÍA DEL ROSARIO; CIMBARO, FRANCISCO STEFANO; LÓPEZ, AYELÉN EMILCE; SIMONIN, ALEJANDRO; SCHARN, AGUSTINA; SILVESTRO, CINTIA; CASTRO, ARACELI; FERRER, PILAR; BELAICH, MARIANO N.; CROTTOGINI, ALBERTO; LOCATELLI, PAOLA; OLEA, FERNANDA D.

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias.; 2022

Introduction: We have recently designed a TBX20-encoding baculovirus (Bv-Tbx20) to be used as a possible therapeutic vector in myocardial regeneration. The transduction of cardiomyocytes in culture with BvTbx20 increased cell proliferation and their supernatant induced angiogenesis. The aim of the present study was to evaluate the effect of the intramyocardial administration of BvTbx20 in sheep with acute myocardial infarction (AMI).Methods and Results: in sheep with experimental AMI, 10^10 copies of BvTbx20 or BvNull were administrated intramyocardially in the infarct border zone. At 7 days post-treatment, BvTbx20 group showed a) the higher expression of the Tbx20 transgene and mitogenic and angiogenic genes than BvNull group, b) more ki67-labeled cardiomyocytes/mm2 (14.37±7.22 vs. 6.32±6.69, p