Host species and geographic origin shape the gut bacterial community of Anastrepha fraterculus larvae

SALGUEIRO, JULIETA; A.L. NUSSENBAUM; MILLA, FABIÁN H.; GOANE, L.; RUIZ, M. J.; BACHMANN, G. E.; VERA M. T.; TSIAMIS, GEORGE; LANZAVECCHIA, S.; SEGURA, D.F.

Congreso; X Meeting of the Fruit Fly Workers of the Western Hemisphere; 2020

The intimate association between host fruit and fruit flies during development envisions that fruit should affect the gut bacterial community hosted by larvae. The present work addresses the effect of host species on gut bacterial diversity in Anastrepha fraterculus larvae considering the potential effect of the geographic origin. We considered 2 host fruit species, peach (Prunus persica) and guava (Psidium guajava), in 2 locations, Concordia (Northeast of Argentina) and Horco Molle (Northwest of Argentina). In each location, 6 trees were randomly selected and 5 infested fruits were collected per tree, conforming 120 samples (2 regions x 2 hosts x 6 trees x 5 fruits). From each fruit, 5 A. fraterculus larvae were extracted, superficially sterilized and dissected under sterile conditions. Middle and posterior gut of 5 larvae were pooled and DNA extraction was performed. After amplifying and sequencing V3, V4 hypervariable regions of 16S rRNA gene by Illumina MySeq, the results of our data analysis revealed that gut bacterial community of larvae is modeled both by host fruit and geographical region. Firstly, larvae collected from guava (G) and peach (P) showed different bacterial profiles evidenced by β-Diversity, α-Diversity (Shannon), Richness (Chao), and OTUs composition. G presented higher values of Shannon and Chao than P. Secondly, gut bacterial communities from Concordia (C) and Horco Molle (HM) did not differed significantly in Shannon or Chao values, but relative abundance pattern analyzed through β-Diversity showed that the bacterial profile differed significantly between C and P. Furthermore, bacterial community of G and P were studied separately and both β-Diversity results showed are affected significantly by geographic origin. In G, Tatumella was surprisingly abundant, followed by Enterobacter, Wolbachia and Gluconobacter. Lactobacillus was exclusively present in larvae from G collected in C. In P Wolbachia was dominant followed by Acetobacter in C, and Enterobacter in HM. Our work confirmed the impact of the host fruit on gut bacterial composition and reveals the strong effect of geographic origin, found even in the same type of host fruit.