High fat diet and chronic stress exposure alter glucydic metabolism, gut microbiota and liver and small intestine histology

MARIA PAULA MARCONE; MARA ROXANA RUBINSTEIN; SOFIA QUIROGA; GABRIEL VALDIVIESO; MIRIAM RUTH WALD; ANA MARÍA GENARO

Mar del plata

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

Sociedad Argentina de Investigación Clínica

In recent decades, overweight and obesity have become a growing health problem. The main risk factors include increased fat intake and exposure to stressful situations. In the last years, gut microbiota (GM) has become increasingly important, contributing to the regulation of energy homeostasis. It has been described that stress and diet could alter the composition of GM, causing dysbiosis and increased intestinal permeability, inducing inflammation.In this context, the aim of this work was to investigate the relationship between high-fat diet (HFD) and chronic exposure to environmental stressors in metabolism, composition of the gut microbiota and histological alterations in liver and small intestine. The investigation was carried out in male C57Bl/6J mice consisting of four experimental groups (n=7): mice fed a normal diet (ND), DN+chronic stress (CS), high-fat diet (HFD) and HFD+CS.We observed a significant increase in body weight, fasting blood glucose and blood glucose overload test in mice fed HFD (p