Thyroid tumor cell-fibroblasts interaction produces extracellular vesicles involved in thyroid tumor aggressiveness

BRAVO MIANA, ROCÍO M.; NEGRETTI BORGA, DANA MARÍA; SOLER, MARÍA FLORENCIA; DE PAUL, ANA L; GILARDONI MÓNICA B.; MONTESINOS, MARÍA DEL MAR; PELLIZAS, CLAUDIA G; DONADÍO, ANA CAROLINA

Buenos Aires

Congreso; XVII Latin American Thyroid Society Congress; 2019

Latin American Thyroid Society Congress

Introduction: Carcinomas are complex societies of mutually interacting cells. Extracellular vesicles (EVs) mediate intercellularcommunication in the tumor microenvironment (TM). Metalloproteinases (MMPs) activity and Galectin-1 (Gal-1) expression in TMregulate tumor-stroma crosstalk leading to tumor progression and aggressiveness. We demonstrated that thyroid tumor cells- fibroblasts(Fb) interactions induce the secretion of MMPs to culture supernatants (CMs) and a migratory phenotype in tumor cells. Objectives:To characterise EVs released by Fb and thyroid cells, and their role mediating a thyroid tumor-promoting environment. Methods:Thyroid tumor cells (TPC-1, 8505c) or thyroid non-tumor cells (NThyOri) were co-cultured with normal human Fb as a simulation ofthe thyroid TM. EVs, obtained by ultracentrifugation of thyroid cells, Fb and thyroid cells-Fb CMs were characterised by TransmissionElectron Microscopy and Western blot. MMPs activity was analysed by zymography in EVs, and in CMs from Fb and NThyOri cellsincubated with EVs. Gal-1 expression was evaluated by RT-qPCR in EVs-stimulated Fb. Results: Isolated and co-cultured cellssecreted EVs to CMs. EVs have the characteristic cup-shaped morphology, size (