Maternal treatment of butyrate during gestation and lactation impacts on maternal metabolism and improves metabolic programming of the offspring

LABIANO, MARINA CECILIA; FALETTI ALICIA GRACIELA; HEINECKE FLORENCIA; FLORES QUIROGA, JEREMÍAS PABLO; MARÍA AGUSTINA MENEGHINI; WHITE VERÓNICA

Mar del Plata

Congreso; LXVII Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2022

Sociedad Argentina de Investigación Clínica (SAIC)

Maternal obesity programs metabolic abnormalities in fetuses that precede a high susceptibility to the development of fatty liver later in life. Butyrate (B), a product of fiber metabolism from intestinal microbiota, improves lipid metabolism and prevents inflammation. We previously observed that maternal oral administration of B preventedmaternal hypertriglyceridemia, fetal overgrowth and liver lipid overaccumulation in a maternal model of overweight in rats. Our aim was to evaluate whether B was able to ameliorate the negative program induced by maternal obesity in the offspring. Methods:Female Wistar rats were fed standard diet (CT rats) or saturated fat-rich-diet (FD rats) for 8 weeks and mated with control males. Vehicle or B (3%) was orally delivered daily during gestation and 3 days per week during lactation (FDB rats). The mothers were euthanizedafter weaning and the offspring at 140 days of life. Maternal liver levels of triglycerides (TG) and cholesterol esters (CE) were assessed by TLC and lipoperoxidation by TBARS assay. Results: We found a decrease in maternal liver weight only in FD group (12% p<0.05 vs CT) and an increase in adipose tissue weight in FD group (120% p<0.1 vs CT), that was not observed in FDB group. The increase in TG and EC content that we had observed at birth persisted in maternal liver from FD (TG: 225%, ChE: 109%) and FDB group p<0.001 vs CT. Maternal liver from FD group presented an increase in lipoperoxidation (228% p<0.05 vs CT), that was not observed in FDB group. In the adult offspring we observed a decrease in body weight in males from the FDB group (16% p<0.01 vs CT). B also prevented the increase in adipose mass in males and females from the FDB group (32% and 10% respectively p<0.05 vs FD). Conclusions: B was able to attenuate the increase in adipose tissue weight and lipoperoxidation in mothers. Also, the adult offspring from theFDB group presented a decrease in adipose tissue weight.