MORPHOLOGICAL CORRELATION BETWEEN THE MAIN SEGMENTS OF THE SEMINIFEROUS TUBULE, DETERMINED BY TRANSILLUMINATION, STRUCTURE AND CELLULAR ISOLATION. PRELIMINARY RESULTS.

VIRGINIA AVENA; FUNES, ABI K; ROCIO AGUERO; MARIA MONCLUS; CONTE, MARIA INES; PAOLA BOARELLI; ESTEFANIA SAEZ LANCELLOTTI; FORNES, MIGUEL W.

MAR DEL PLATA

Congreso; REUNION CONJUNTA SAIC - SAI - SAFIS - 2022; 2022

SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA

The seminiferous epithelium (ES), of the seminiferous tubule (TS), goes through various epithelial stages (ES) forming a cycle of the germinal epithelium, before delivering a finished sperm. These stages have their own rhythm in each species, temporally and histologically. In turn, in the TS - isolated and intact - 3 main zones are observed by transillumination: clear (Z1), intermediate (Z2) and dark (Z3) that are associated with the ES cycle. Each cycle of the ES results from a still unknown regulation. The study of cycles involves their correct isolation and characterization. In the rabbit, the EE (VIII Correlative Cycles) have been described, but the corresponding zones have not been isolated or characterized, and even less their regulation. The objective of the work was to relate zones and EE by fine-tuning the isolation of the main zones of the ST, verifying the success by isolating specific germ cells of the stage. New Zealand adult rabbit testes were used. One half was fixed in Bouin and the other was decapsulated, releasing the TS in a Petri dish. They were incubated 10 minutes/37°C with collagenase. Under magnification and transillumination, the loose TS were separated into Z1, Z2 and Z3. Some were fixed in Bouin and others were subjected to "squash" detaching the cells from the ES by fixing them with p-F 4%. Testis, isolated TS and cells were processed for light and electron microscopy. The morphological correlation between TS zones and ES cycles indicates that stages III and IV correspond to Z1, V to VII to Z2 and I, II and VIII to Z3. The proportion of germ cells obtained by squash from each zone corresponds to the cell types observed in the histological analyses, a higher proportion of round and elongated spermatids in Z2, more spermatocytes and no sperm in Z1, inversely to what was seen in Z3 (p< 0.05). These observations allow us to use transillumination to isolate EE and advance in the study of their regulation, within the areas of the rabbit TS.