AN INCREASE OF GRANULOCYTES AND LEUCOCYTES EXPRESSING S100A9 IN PATIENTS WITH CERVICAL CANCER IS ASSOCIATED WITH A WORSE PREDICTIVE RESPONSE

YANINA VERÓNICA LANGLE; PABLO DAMIÁN CRESTA MORGADO; DENISE BELGOROSKY, ; FLORENCIA GUERRA,; PABLO MARENCO, ; PABLO MENÉNDEZ, ; VALERIA CÁCERES, ; ANDREA AGUILAR, ; MARCELA OSTOJICH, ; LAURA LAY, ; ANA MARIA EIJAN; EDUARDO OMAR SANDES

MAR DEL PLATA

Congreso; Reunion Anual de Sociedades de Biociencias SAIC-SAI-SAFIS; 2022

Cervical cancer (CCa) is the second most common type of cancer affecting women. Leukocytosis is related with treatment failure and with the induction and accumulation of myeloid derived suppressor cells (MDSC) in peripheral blood. S100A9 is a useful marker to identify MDSC in whole blood. In this study, we analysed in blood of patients with locally advanced CCa (Pts) and in healthy donors (CRLs), the number of total leukocytes, monocytes (CD14+) and granulocytes (CD15+) that express S100A9.Peripheral blood from CRLs and Ptes with CCa (stage IB3-IVA) treated between 2018-2021 with concurrent chemoradiotherapy and brachytherapy were collected. Leukocytes were isolated and stained for S100A9, CD14 and CD15 and then analysed by flow cytometry. The predictive value of S100A9+ cells between Pts and CRLs was evaluated and associated with therapeutic response.Results: An increase in S100A9+ leukocytes and granulocytes were observed in peripheral blood from Pts before treatment compared to CRLs (p=0.0003 and p=0.0005, respectively). Tumor response was dichotomized as complete or insufficient response (persistence + progression), associated to S100A9+ cells. Using a logistic regression model, we observed that the percentage of leukocytes and granulocytes S100A9+ exhibited a trend close to significance of being associated with tumor response [Leukocytes odds ratio (OR): 0.908, 95% confidence interval (CI): 0.819-1.006, p=0.06; Granulocytes OR: 0.907, 95% CI: 0.821-1.002, p=0.057]. In this way, it represents that 1% increase in S100A9+ leukocytes or granulocytes are associated with a 9% reduction of having a complete response.Conclusions: Pts with locally advanced CCa presented an increase in total leukocytes and granulocytes that express S100A9. The association between S100A9+ leukocytes and granulocytes with tumor response might suggest that Pts with greater levels of these cells could define a population with higher risk of therapeutic failure.