BECAS
PALMA NicolÁs Francisco
congresos y reuniones científicas
Título:
STUDY OF THE EFFECT OF GLYCEROL (GLY) ON THE SURVIVAL AND MIGRATION OF HEPATOCELLULAR CARCINOMA CELLS TREATED WITH SORAFENIB (SFB)
Autor/es:
CHARES LAURA G; CARMELI EUGENIA; PALMA NICOLÁS FRANCISCO; LIVORE, VERÓNICA INÉS; FRATTINI MAGALÍ; FERRETTI, ANABELLA, C; COMANZO, CARLA G; OVIEDO BUSTOS, LUCÍA; QUIROGA, ARIEL DARÍO; ALVAREZ, MARÍA DEL L.; CEBALLOS MANCINI, MARÍA PAULA
Lugar:
Mar del Plata
Reunión:
Congreso; REUNIÓN ANUAL 2022 DE LA SOCIEDAD ARGENTINA DE FISIOLOGÍA (SAFIS); 2022
Institución organizadora:
Sociedad Argentina de Fisiología
Resumen:
SFB is used as a first-line therapy for HCC; however, its efficacyis limited and better therapies to prolong survival are still lacking.Combination therapy represents a major research direction for HCCtreatment. Our group reported antiproliferative and proapoptotic effects of GLY administration in an in vivo model of liver carcinogenesis. Aim: to analyze whether GLY enhances the sensitivity of HCCcells to SFB in vitro. Methods: 2D and 3D cultures from Huh7 cellswere treated for 72 h with different concentrations of SFB or GLY toobtain dose-response curves. Alternative, cell cultures were treatedfor 72 h with SFB (2D: 2μΜ; 3D: 4μΜ), GLY (2D: 1μΜ; 3D: 2μΜ),SFB+GLY, or DMSO (control group). Then, cell viability (2D: MTT;3D: acid phosphatase; IC50), proliferation (3D: spheroid growth -volume 72h/0h) and migration (2D: wound healing; 3D: spheroidmigration) were assayed. Results: SFB and GLY reduced 2D and3D cell cultures viability in a dose-dependent manner compared withcontrol group. The 3D cell culture was less sensitive to drugs thanthe 2D cell culture (IC50: SFB 2D: 3.4μΜ, 3D: 13.7μΜ$; GLY 2D:2.4μΜ, 3D: 10μΜ$). In 3D cell cultures, both drugs induced dose-dependent growth inhibition. Cell cultures treated with SFB+GLYshowed a greater decrease in viability compared to cultures treatedwith SFB or GLY (2D: SFB -23%*, GLY -22%*, SFB+GLY -56%*#&;3D: SFB -16%, GLY -1%, SFB+GLY -42%*#&). Treatment of 3D cellcultures with SFB+GLY decreased spheroid growth compared to individual treatments (SFB -25%*, GLY -11%*, SFB+GLY -35%*#&).SFB+GLY treatment produced the greatest reduction in cellular migration in 2D and 3D cultures. $p
