ANTIPROLIFERATIVE EFFECT OF COLEUS NEOCHILUS EXTRACT ON HUMAN BREAST CANCER CELL LINES

MAYORA JUSTEL C; ESANDI MC; VILLAMIL C; GOMILA C; SALVADOR G; BOUZAT C; LUTHY I; ALZA N; BRUZZONE A

Mar del Plata, Buenos Aires, Argentina

Congreso; Reunión Anual de Sociedades de Biociencias; 2022

Sociedad Argentina de Investigación Clínica

ANTIPROLIFERATIVE EFFECT OF COLEUS NEOCHILUS EXTRACT ON HUMAN BREAST CANCER CELL LINESCarla Luciana Mayora Justel1, María del Carmen Esandi1,2, Carlos Villamil2, Carolina Gomila1, Gabriela Salvador1,2, Cecilia Bouzat1,2, Isabel Lüthy3, Natalia Alza1 and Ariana Bruzzone11 Instituto de Investigaciones Bioquímicas Bahía Blanca-CONICET.2 Departamento de Biología Bioquímica y Farmacia -UNS.3 Instituto de Biología y Medicina Experimental-CONICET. The Plectranthus species have a rich diversity of ethnobotanical uses. Nevertheless, the most frequently use of these species is for their medicinal properties. This work focuses on Coleus neochilus(Schltr.) Codd, also known as “boldo rastrero” (BR), a specie closely akin to the genus Plectranthus, and its effect on human breast cancer cell lines. A crude ethanolic extract of leaves of BR was prepared. BR incubation decreased cell viability in MCF-7, MDA-MB-231 and T47D cancer cell lines. The extract concentration that caused a 50% inhibition in cell viability (IC50) was lower for tumor cells than for nontumor cells (about 45, 19, 26 vs 50 for MCF-7, MDA-MB-231, T47D and MCF-10A respectively). At 10 μg/ml, BR reduced cell proliferation in all tumor cells but not in non-tumor cells (i.e., 45% reduction in MCF-7, p