INFECTION WITH T. cruzi AND THE GENERATED IMMUNOLOGICAL MEMORY INHIBIT TUMOR GROWTH IN C57BL/6 MICE CHALLENGED WITH THE B16-F10 CELL LINE

KAUFMAN, CINTIA DANIELA; FARRÉ CECILIA; BISCARI, LUCÍA; HUHN, VICTORIA; PÉREZ, ANA ROSA; ALLOATTI ANDRÉS

MAR DEL PLATA

Congreso; REUNIÓN CONJUNTA SAIC SAI&FAIC SAFIS 2022; 2022

SOCIEDAD ARGENTINA DE INMUNOLOGÍA

Spontaneous regression of tumor is often associated with infections. To evaluate the antitumor effect of the infection with Trypanosoma cruzi and the associated immunological memory, C57BL/6 female mice were randomly divided into four groups: (G1) MEMORY received an intraperitoneal (IP) dose of 10,000 trypomastigotes (day 0) and mice were treated with Benznidazole (BZ). To boost the response, this scheme was repeated two months later; (G2) INFECTION received an IP injection of 500 trypomastigotes (day 110); (G3) BENZNIDAZOLE was not infected but was treated with BZ as G1; (G4) TUMOR was neither infected nor received BZ. All groups were challenged subcutaneously with B16-F10 tumor cells (day 125). Parasitemia and tumor growth were monitored. No differences were observed in the tumor volume and survival between G3 and G4, and thus G3 was excluded from the analyses. From day 7 post-inoculation (PI), differences in tumor volume were found among groups (G2 vs G4 - days 7 and 11-18 PI. – p