ABSENCE OF LEUKOCYTE SPECIFIC PROTEIN 1 (LSP1) ALTER ACTIVATION AND PROLIFERATION OF CD4+ AND CD8+ T LYMPHOCYTES

LOPEZ MENICHETTI, GUADALUPE ANAHÍ; PASCUAL, MARÍA MERCEDES; RUIZ MORENO, FEDERICO; CRESPO, MARÍA INÉS; MALETTO, BELKYS A; MORÓN, GABRIEL; DHO, NICOLÁS DANIEL

Mar del Plata

Congreso; Reunión Anual de Sociedades de Biociencias SAIC-SAI&FAIC-SAFIS; 2022

Sociedad Argentina de Inmunología

Leukocyte-specific protein 1 (LSP1) is a 52 kDa cytoplasmic F-actin binding phosphoprotein expressed in all human and murine leukocytes and endothelial cells. We have previously shown that Lsp1-/- mice have an impaired CTL response after antigen exposure, with Lsp1-/- dendritic cells (DCs) failing to induce a strong CTL response in vivo and a lower CD4+ T cell-mediated response.In order to study the role of LSP1 in T cell functionality, we evaluated the capacity of Lsp1-/- CD4+ and CD8+ T lymphocytes to activate and proliferate in vitro. T cells were purified from spleen of Lsp1-/- and WT mice by cell sorter after labeling splenocytes with anti-CD8β (to purify T CD8+ cells), anti-CD11b and anti-CD4 antibodies to purify T CD4+ lymphocytes. Sorted T cells were then stained with eF670 proliferation dye and finally cocultured with Ab anti-CD28 in a well plate sensitized with anti-CD3. Three days later, a significantly lower percentage of activated cells expressing CD69 (p