ASTROCYTIC INSULIN SIGNALING AND INFLAMMATION IN EXPERIMENTAL ALZHEIMER’S DISEASE

BENTIVEGNA, MELISA; AMAL GREGOSA; ANGELES VINUESA; CARLOS POMILIO; JESSICA PRESA; FLAVIA SARAVIA; BEAUQUIS, JUAN

Honolulu

Congreso; ISN-APSN 2022 Meeting; 2022

ISN

Insulin resistance (IR) and chronic inflammation are associated with the development of cognitive disorders and neurodegenerative diseases such as Alzheimer´s (AD). However, it is not clear whether there is a causal link between these factors, which one appears earlier in the pathology or if either one of them is triggered by the increasing circulating levels of Aβ or amyloid deposits in early AD. Our objective was to study the metabolic and inflammatory status of a model of AD, the PDAPP-J20 mouse at the age of 8 months. We also treated a WT group with a high fat diet (HFD) as a positive control for IR. Our hypothesis was that in early stages of AD, the brain develops IR with astrocytes showing reactivity and impaired insulin signaling. Final body weight, glycemia and insulinemia were not affected by genotype or HFD. The open-field test showed an anxious-like behavior in transgenic and in HFD-fed mice. Insulin signaling measured by pAkt/Akt ratio was decreased in the hippocampus of AD mice (p