Perigestational alcohol consumption compromises mouse placental growth and development at term associated to altered blastocyst´s trophectodermal quality

GUALDONI G; BARRIL C; JACOBO P; SOBARZO C; CEBRAL E

Bogotá

Simposio; SLIMP; 2022

SLIMP

Objectives: Normal placentation, critical for fetal growth and postnatal health, depends on trophoectodermal development duringperiimplantation. Gestational alcohol ingestion leads to gestational complications, early miscarriage, and placental anomalies. However,human alcohol consumption prior to and up to the first weeks of gestation on the mature placenta and early trophoblastic etiology receivedlittle attention.We aimed to analyze potential placental morphology-growth anomalies at term and their relationship with trophoectodermal defects fromblastocyst after perigestational alcohol intake up to preimplantation.Methods: treated female mice (TF) were administered with ethanol 10% in drinking water for 15 days before and up to day 4 of gestation(D4, preimplantation), or pregnancy continued without ethanol until day 18 (D18). Control females (CF) consumed water. At D4,trophoectodermal-dependent morpho-functional processes (blastocyst cavitation and expansion) (inverted phase-contrast microscopy),morphogenesis of intercellular adherent (AJ) and tight junctions (TJ) (immunofluorescence, confocal), proliferation (Tarkowski), and themitotic/apoptosis (TUNEL) indexes of blastocyst trophoectoderm were performed. At D18 histological (H&E) and morphometrical (PAS, ImageJ) analyses were conducted in mature placentas.Results: At D18, TF-placental weight was reduced, but fetus/placental index was increased vs CF (p