Delayed implantation site development and impaired decidual angiogenesis via VEGF-KDR system disruption during mouse gastrulation following perigestational alcohol ingestion

BARRIL C; GUALDONI G; JACOBO P; SOBARZO C; CEBRAL E

Bogotá

Simposio; SLIMP; 2022

SLIMP

Objectives: Perigestational alcohol consumption up to organogenesis produces embryo growth restriction and defective maternalvascularization by reduced uNKs and VEGF expression.We aimed to determine whether perigestational alcohol intake up to gastrulation produces early delayed growth of implantation sites (IS)and earlier alters decidual angiogenesis, uNK cell populations, and VEGF-KDR-MMP-9 system.Methods. Ethanol 10% was administered to female mice (TF) for 17 days before and up to days 8 and 8.5 of gestation. Free-ethanol waterwas administered to control females (CF). Histology (H-E, PAS), DBA-histochemistry, immunohistochemistry (IHC), immunofluorescence (IF),TUNEL, and morphometry (ImageJ) were performed in D8-8.5-IS to analyze the vascular decidual tissue.Results. In TF, the percentage of delayed IS (TS10a+ab) increased while advanced IS (TS10b+TS11, Theiler Scale) diminished at D8-8.5 vs CFs.With histological alterations (H-E) and low extracellular matrix deposition (PAS), TF-IS area decreased in TS10b stage and at D8 vs CF-IS.Expansion of decidual vascular lumen/IS area was reduced in D8.5-IS from TF vs CF (p