SHORT AND LONG-TERM ASSESSMENT OF NOISE EXPOSURE ON HIPPOCAMPAL OXIDATIVE STRESS IN ADOLESCENT FEMALE AND MALE RATS

BUJÁN, GE; SERRA, HA; MOLINA, SJ; GUELMAN, LR

Virtual

Congreso; Reunión Conjunta Biociencias 2021; 2021

Asociación Argentina de Farmacología Experimental

Previous studies from our laboratory have shown that noise exposure was able to trigger behavioral and neurochemical alterations in the Central Nervous System (CNS) of adolescent male rats when evaluated at short-term, whereas results in females have not been obtained yet. In addition, long-term measurements have not been performed. Therefore, the aim of the present work was to investigate the effects of noise exposure in the hippocampus (HC) of adolescent female and male rats on biochemical parameters evaluated at short and long-term. Male and females PND28 Wistar rats were separated into different cages and at PND 33 a subgroup was exposed to noise (2h, 95-97 dB). HC was dissected at short (PND 33) and long­term (PND 39) to assess reactive oxygen species (ROS) levels and catalase activity (CAT). Results showed an increase in ROS levels in females (sham: 0.004±0.001; noise: 0.123±0.005) and males (sham: 0.019±0.004; noise: 0.054±0.003) and an increase in CAT activity only in males (sham: 0.0004±0,0002 noise: 0.001±0.0001) when evaluated at short term. In contrast, long-term results showed a decrease in CAT activity in females (sham: 0.001±0.0004; noise: 0.0003±0.0007), whereas no significant differences were found in males. No differences were found in either group in ROS levels.These results suggest that noise exposure may induce short-term changes in oxidative markers that seem to disappear at long-term and to be sex-specific. In conclusion, adolescence seems to be a period of vulnerability to different stimuli capable of generating oxidative imbalance in the hippocampus, which could underlie some of the behavioral changes previously observed.