INVESTIGADORES
ZANETTI Maria Eugenia
congresos y reuniones científicas
Título:
Common bean WRKY and bHLH TFs differentially regulated in strain-specific symbiosis
Autor/es:
ELSZTEIN C, BEKER MP, PELTZER-MESCHINI E, ZANETTI ME, BLANCO F, AGUILAROM
Lugar:
Mar del Plata, Buenos Aires, Argentina
Reunión:
Congreso; XLIII Reunión anual de SAIB; 2007
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
Resumen:
Common beans ( ) establish symbiosis with the
soil rhizobia resulting in nitrogen fixation within a newly formed
organ, the nodule. We have previously shown coevolution of the
symbiotic partners resulting in a preference in nodulation by strains
of rhizobia from the same geographical region. In order to identify
genes of the plant involved in this strain selection, 11502 ESTs were
generated from root hairs of Mesoamerican common beans
inoculated with . One hundred and ninety genes for
signal perception and transduction, transcriptional activation and
hormone responses, out of 2108 independent sequences we
examined, were selected and spotted in a macroarray. Membranes
were hybridized with cDNAof roots inoculated with different
strains to identify genes differentially induced in the more efficient
interaction. Two of the differentially identified genes that were
confirmed by qRT-PCR are predicted to encode transcription factors
belonging to the WRKY and helix-loop-helix family, involved in
pathogen perception and cell differentiation respectively. Transcript
levels of these genes in response to different strains of rhizobia and
regulation by plant hormones were analyzed by qRT-PCR.
Understanding competitiveness and strain specificity is of vital
importance to develop inoculants that can out-compite the
indigenous strains present in the soil.