Anti-inflamatory effect of archaeolipid nanoparticles on human endothelial cells

CHARÓ N L,; JEREZ, HORACIO; TATTI, SILVIO; ROMERO, EDER LILIA; SCHATTNER M

Congreso; Reunión Conjunta SAIC?NANOME.ar ?2021; 2021

Background: New generation of liposomes called archaeosomes (ARC) are nanoparticles (NPs) made of archaeolipids employed as drug delivery systems that exhibit high structural stability. So far there are no reports on the effects of ARC on human vascular endothelium, crucial data for their eventual clinical use.Aim: The present study investigated the effects of archaeosomes on human umbilical vein endothelial cells (HUVECs) in physiological conditions and under inflammatory conditions and compared it with conventional liposomes. Methods: HUVECs were non-stimulated or stimulated with LPS, Pam3CSK4, E. coli, TNF-α or IL-1 β in the absence or presence of nanoparticles. We analyzed cell viability and apoptosis (acridine orange/ethidium bromide), cell proliferation (EdU assay), expression of ICAM-1 and E-selectin (cytometry), secretion of IL-6 and von Willebrand Factor (vWF) (ELISA), mRNA expression of ICAM-1 and vWF (RT-qPCR), uptake of NPs (cytometry and confocal microscopy) and signaling pathways (cytometry and western blot). Results were analized by ANOVA followed by Bonferroni test (p