Design and development of nanocarriers for the optimization of radio-chemosensitization strategies.

GIANNONI F; LLOYD R; CERDA MB; MILENA B; IEZZI ME; MYKHAYLYK O; PODHAJCER O; PLANK C; POLICASTRO L

Mar del Plata

Congreso; 51 Annual Meeting Argentine Society for Biochemistry and Molecular Biology; 2015

Nonviral gene vectors, such as liposomes, are widely used for gene delivery due to their low immunogenicity, biocompatibility, and manipulable synthesis and modification. On the other hand, it is proved that magnetic polyethyleneimine complexes (MPD) enhance in vitro and in vivo transfection efficiency with low cytotoxicity. For all this reasons, the aim of this work is to create a magnetoliposome (ML) that can be magnetically mobilized into the cells surface so that the in vitro transfection could be enhanced. SO-Mag5 magnetic nanoparticles (MNP) were used to form MPD with different Fe/DNA relations (0,2:1; 0,4:1; 0,75:1; 1:1; 3:1) using a fixed N/P relation of 10. We have determined the post-formation MPD hydrodynamic diameter and polydispersity index and visualized its complexation in agarose gel. We chose Fe/DNA 3:1 relation to encapsulate the MPD in negatively charged lipid films to form the ML; the hydrodynamic diameter and polydispersity index was determined and we proved different separation methods to obtain pure ML. Also magnetofection (MF) was performed in two colorectal cancer cell lines (HCT116 and Caco-2).Results show that particle size of ML were accurate for in vitro transfection (160 nm). Luciferase activity was lower for Caco-2 cell line and the highest MF efficiency was obtained for the supernatant of centrifugated ML in both lines. We think that this ML is a potential DNA/iRNA nanocarrier to use in anticancer therapies.