EXPERIMENTAL MODEL OF NUTRITIONAL OBESITY EVALUATION OF ANTHROPOMETRIC, NUTRITIONAL AND METABOLIC PARAMETERS

ALFONSO JO; LOPEZ M; DELGADO SM; PEREZ CHACA V; CIMINARI E; BIAGGIO, V.; SALINAS E; GOMEZ N; NAVIGATORE FONZO LS; ANZULOVICH AC

MODALIDAD VIRTUAL

Congreso; XXXIX REUNION CIENTIFICA DE LA SOCIEDAD DE BIOLOGIA DE CUYO; 2021

Understanding that the cause of Obesity (OB) is multifactorial, including genetic, environmental and dietary factors, amongwhich, high-calorie diets play a central role in the development of the disease, and that OB in adults increases the risk ofassociated neurodegenerative diseases with age, we designed a nutritional model of OB in adult rats, as part of the IMIBIOSL institutional project (PUE-013), which studies OB as a predisposing disease to the development of chronic age-associateddiseases and the search for early biomarkers with predictive potential. In this framework, the particular objective of thepresent work was to evaluate the effects of a rich in saturated fat diet on different anthropometric, nutritional and metabolicparameters. For this, male Wistar rats were weaned at 21 days of age, and fed a normocaloric diet (NC), containing 366 Kcalof lipids / Kg diet, then, at the 2-mo-age, they were randomly separated, and fed: one group, with the NC diet (control, NCgroup) and the other, with a diet high in saturated fat (1570.7 Kcal of margarine / Kg diet, HFD group), for the next 14 weeks.The animals were kept under 12h-light: 12h-dark and 22-24 ° C, with water and food ad-libitum. The anthropometric profileincluded the evaluation of: body weight, body mass index (BMI), weight gain and Lee's index, the nutritional parameters:energy intake and feeding efficiency, all of them were determined weekly, throughout the entire treatment period. Themetabolic parameters included: glucose (G), triglycerides (TG), total cholesterol (TC), HDLc, and LDLc + VLDLc levels,and were determined at the end of the experiment, in the serum of both, NC and HFD groups, using commercial kits.Statistical differences between groups and throughout the treatment period were analyzed by two-way ANOVA, followed byBonferroni post-hoc test, with p