The Isothiocyanate sulforaphane induces RBOHD-dependent reactive oxygen species production and enhances expression of plant biotic and abiotic related genes

PERK ENZO A.*; ARRUEBARRENA DI PALMA ANDRÉS*; CARBONI MARTIN F., GARCÍA- MATA CARLOS, TÖR MAHMUT AND LAXALT ANA M.

Santa Fe

Congreso; RAFV; 2021

Sulforaphane (SFN) is an isothiocyanate-type phytomolecule present in crucifers, which is mainly synthesized against biotic stress. In animals, SFN incorporated in the diet has anticancer properties, and the mechanism of action and signaling were described. However, little is known about SFN signalling and mechanism of action in plants. Our goal in the present study was to elucidate components of the SFN signaling pathway, particularly the production of hydrogen peroxide (H2O2), and its effect on the transcriptome using the RNAseq technique. In Arabidopsis SFN cause H2O2 production exclusively through the action of the NADPH oxidase RBOH isoform D. On the other hand, regarding the SFN effect on the transcriptome, we obtained a large increase in the expression of heat shock proteins (HSP) genes and also genes associated with response to oxidative stress. As a confirmation of the relationship between SFN and the response to biotic stress, many genes related to biotic stress response were upregulated. In addition, SFN increases the levels of transcripts related to the response to abiotic stress, as well as related to various phytohormones.Taken together, these results indicate that SFN induces an oxidative burst, leading to signaling events and gene expression. This oxidative burst may cause a cellular imbalance, with the increase of the expression of genes such as heat shock proteins to restore cellular homeostasis. In our analysis we obtained many upregulated genes that codifies for possible components of the signalling pathway and putative effectors.