INVESTIGADORES
PAGNUSSAT Luciana Anabella
congresos y reuniones científicas
Título:
Iron deficiency-induced responses in Arabidopsis thaliana roots: role of ethylene
Autor/es:
NEGRI. P., CABALLERO V., DÍPPOLITO S., MASSAZZA D., RAMIREZ L., SCHROTT G., TAGLIOTTI M., PAGNUSSAT L, PINEDO M. Y LAMATTINA L.
Lugar:
Rosario
Reunión:
Congreso; XLII Anual Meeting SAIB; 2006
Institución organizadora:
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Resumen:
A
low iron availability in soil generates a series of plant root
responses
that lead to the increase of iron uptake. Fe+3-
reductase
(FRO2) under the control of the transcription factor
(FIT1)
and morphologic changes in roots have been described to
be
induced by iron deficiency. Since different reports have
involved
nitric oxide (NO) and ethylene in plant iron metabolism,
we
decided to study the role of ethylene and its crosstalk with NO
in
plant root responses to iron deficiency. Plants of Arabidopsis
thaliana grown in
presence or absence of iron were treated with
ethephon
(ethylene donor) or CoCl2 (ethylene
synthesis
inhibitor).
The morphologic analysis of the roots showed that
depletion
of endogenous ethylene completely inhibited roots
hairs
development. NO production in roots was increased by
ethylene
treatment and decreased when adding CoCl2.
Beside,
FRO2
and FIT1 expression were evaluated by semiquantitative
RT-PCR.
Results indicated that, while CoCl2 did not
affect the
FRO2
and FIT1 mRNA levels in presence or absence of iron, the
ethylene
addition decreased those levels. In conclusion, ethylene
would
play a role in root hairs development and NO production
and,
at less extent, would also influence FRO2 and FIT1
expression
as well as FRO2 activity.