VOLATILE ANAESTHETICS AND MITOCHONDRIA DAMAGE: ALTERATIONS ON CHAIN RESPIRATORY AND KREBS CYCLE.

JOHANNA ZUCCOLI; MARIA DEL CARMEN MARTINEZ; SILVINA FERNANDA RUSPINI; ALCIRA BATLLE; BUZALEH, ANA MARÍA

Bordeux

Congreso; INTERNATIONAL CONGRESS ON PORPHYRINS AND PORPHYRIAS 2017; 2017

Heme deficiency by a reduced synthesis or an accelerated catabolism would trigger severe cell damage.Previously we demonstrated that porphyrinogenic agents affected several brain metabolisms includingrespiratory mitochondrial chain in encephalon CF1 mice. The aim was to study the effects of volatileanaesthetics and other xenobiotics on the activity of I to IV complexes of respiratory chain in encephalonof a mouse model of Acute Intermittent Porphyria. Male knockout mice (PBGD-/-) were treated withIsoflurane (2 ml/kg), Sevoflurane (1,5 ml/kg), ethanol (30%), allylisopropylacetamide (AIA, 350 mg/kg) andVeronal (167 mg/kg), or starved 24 hours. Veronal increased the activity of II-III complexes (370%, p