Bacterias lácticas y Bifidobacterias de un criadero de Rana catesbeiana de Córdoba: Evaluación de sus características benéficas.

GABRIELA MONTEL MENDOZA; CESAR E. ALE; SERGIO E PASTERIS; MARIA CLAUDIA OTERO; MARTHA BUHLER; MARIA ELENA FÁTIMA NADER

Tafi del Valle

Jornada; XXVI JORNADAS CIENTIFICAS DE LA ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN.; 2009

ASOCIACIÓN DE BIOLOGÍA DE TUCUMÁN.

LACTIC ACID BACTERIA AND BIFIDOBACTERIA FROM A BULLFROG HATCHERY OF CORDOBA: EVALUATION OF THE BENEFICIAL CHARACTERISTICS. G. Montel Mendoza1; C.E. Ale1; S.E. Pasteris1; M.C. Otero1; M. Bühler1; M.E. Nader-Macías2.1INSIBIO-CONICET-UNT, 2CERELA-CONICET. Tucumán. fnader@cerela.org.ar   Rana catesbeiana is the most appropriate specie for raniculture, where diseases such as red leg syndrome (RLS) contribute to animal mass mortality with a high impact in the production costs. The use of probiotics is becoming increasingly popular in aquaculture, being Lactic Acid Bacteria (LAB) the most suitable microorganisms for this purpose. In this work we evaluated the beneficial properties (production of antagonistic compounds and surface properties) of 52 LAB and 5 Bifidobacterium sp. isolated from a hatchery of Córdoba and their differences with the LAB isolated from a Northwest hatchery. Results showed that all the strains inhibited at least one of the assayed pathogens by organic acid (agar well diffusion method). 16 strains were found to produce H2O2 in a specific medium (MRS+TMB+peroxidase). Only one strain produced a bacteriocin active against S. epidermidis and L. monocytogenes. Surface properties assays showed that most of the strains were hydrophilic. However, 8 strains exhibited a high hydrophobicity percentage, while 5 were autoagreggative. Bifidobacteria presented hydrophilic properties and only one showed antagonist activity. LAB isolated from a Northwest hatchery have similar inhibitory and surface properties than those from Córdoba. However, the percentage of H2O2-producing strains was higher. The results obtained allow us to select some potential probiotic strains to be used in different aquaculture areas according to the biodiversity found.