Co-immunoprecipitation of Plant Receptor Kinases

MARÍA LAURA BARBERINI; JORGE MUSCHIETTI

Plant Receptor Kinases

Humana Press

Lugar: New York; Año: 2017; p. 109 - 112

In order to comprehend the function of a particular protein, identification of the interacting protein partners is a useful approach. Co-immunoprecipitation (Co-IP) is employed to test physical interactions between proteins. Specific antibodies or antibodies against tagged versions can be used to immunoprecipitate the proteins. In this chapter, we describe a method to carry out Co-IP using recombinant membrane proteins expressed in yeast microsomal fractions