Analysis of expression gradients of developmental regulators in Arabidopsis thaliana roots

ERCOLI, MA FLORENCIA; VENA, RODRIGO; GOLDY, CAMILA; PALATNIK, JAVIER F; RODRIGUEZ VIRASORO, RAMIRO ESTEBAN

Methods in Molecular Biology - Morphogen Gradients

Humana Press

Lugar: New York; Año: 2018;

The regulatory mechanisms involved in plant development include many signals, some of them acting asgraded positional cues regulating gene expression in a concentration-dependent manner. These regulatorymolecules, that can be considered similar to animal morphogens, control cell behavior in developing organs.A suitable experimental approach to study expression gradients in plants is quantitative Laser ScanningConfocal Microscopy (LSCM) using Arabidopsis thaliana root tips as a model system. In this chapter, weoutline a detailed method for image acquisition using LSCM, including detailed microscope settings andimage analysis using FIJI as software platform.