Micropropagation of Codiaeum variegatum (L.) Blume and regeneration induction via adventitous buds and somatic embryogenesis.

RADICE S.

Protocols for in vitro propagation of ornamental plants

The Humana Press/Springer

Lugar: Clifton, New Jersey; Año: 2008;

&amp;amp;lt;!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:PL; mso-fareast-language:PL;} h1 {mso-style-next:Normal; margin:0cm; margin-bottom:.0001pt; line-height:200%; mso-pagination:widow-orphan; page-break-after:avoid; mso-outline-level:1; font-size:12.0pt; font-family:"Times New Roman"; mso-font-kerning:0pt; mso-ansi-language:EN-GB; mso-fareast-language:EN-US; mso-bidi-font-weight:normal;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --&amp;amp;gt; <!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Codiaeum variegatum (L) Blume cv. “Corazon de oro” and cv.“Norma” are successfully micropropagated with explants taken from newly sprouted shoots. The establishment and multiplication steps are followed by adding 1 mg/L BA or 1 mg/L IAA and 3 mg/L2iP to the MS medium, which is dependent on the cultivar. Adventive organogenesis and somatic embryogenesis are induced from leaf explants taken from in vitro buds of croton. On leaf sections of “Corazon de oro” cultured in vitro, 1 mg/L BA stimulates continuous somatic embryos development and induces some shoots too. Replacing BA with 1 mg/L TDZ induces up to 100% bud regeneration in the same explants. On the other hand, leaf sections of C. variegatum cv. Norma are unable to differentiate somatic embryo without adding 1 mg/L TDZ in the MS basal medium. Incipient callus is observed after 30 days of culture and then, subculture to MS containing 1 mg/L BA allows the same process to show on the “Corazon de oro” cultivar. Somatic embryos show growth arrest that is partially overcome by transfer to hormone-free MS basal medium amended with activated charcoal. Root induction occurs on the MS basal medium supplemented with 1 mg/L IBA. Plantlets in the greenhouse has true-to-type variegated leaves.