Cardiac Natriuretic Peptide Profiles in Chronic Hypertension by Single or Sequentially Combined Renovascular and DOCA-Salt Treatments

CERRUDO CS; CAVALLERO S *; RODRIGUEZ FERMEPIN M; GONZÁLEZ GE; DONATO M; KOUYOUMDZIAN N; GELPI RJ; HERTIG CM; CHOI M; FERNANDEZ BE

Natriuretic Peptides in Cardiovascular Pathophysiology

Frontiers in physiology

Año: 2022; p. 25 - 36

The involvement of natriuretic peptides was studied during the hypertrophic remodelingtransition mediated by sequential exposure to chronic hemodynamic overload. Weinduced hypertension in rats by pressure (renovascular) or volume overload (DOCAsalt)during 6 and 12 weeks of treatment. We also studied the consecutive combinationof both models in inverse sequences: RV 6 weeks/DS 6 weeks and DS 6 weeks/RV6 weeks. All treated groups developed hypertension. Cardiac hypertrophy and leftventricular ANP gene expression were more pronounced in single DS than in single RVgroups. BNP gene expression was positively correlated with left ventricular hypertrophyonly in RV groups, while ANP gene expression was positively correlated with leftventricular hypertrophy only in DS groups. Combined models exhibited intermediatevalues between those of single groups at 6 and 12 weeks. The latter stimulus associatedto the second applied overload is less effective than the former to trigger cardiachypertrophy and to increase ANP and BNP gene expression. In addition, we suggest acorrelation of ANP synthesis with volume overload and of BNP synthesis with pressureoverload-induced hypertrophy after a prolonged treatment. Volume and pressureoverload may be two mechanisms, among others, involved in the differential regulationof ANP and BNP gene expression in hypertrophied left ventricles. Plasma ANP levelsreflect a response to plasma volume increase and volume overload, while circulatingBNP levels seem to be regulated by cardiac BNP synthesis and ventricular hypertrophy.