Independent transcriptomic and proteomic networks reveal common differentially expressed chaperone and interactor genes during tomato cv. Micro-tom fruit ripening

BERTERO, VALENTINA GOYTIA; PRATTA, GUILLERMO R.; ARCE, DÉBORA P.

Plant Gene

ELSEVIER

Año: 2021

2352-4073

Heat Shock Proteins (HSPs) are a superfamily of chaperones that have been characterized in different organisms.In plants, HSPs contribute to abiotic stress responses and development. The aim of this work was to perform anintegrative analysis using bioinformatic and inferential approaches based on protein-protein interactions (PPI)network building, using transcriptomic and proteomic public data from Solanum lycopersicum cultivar (cv.)Micro-Tom during fruit ripening. We were able to identify specific chaperone networks for each maturing stageat both transcriptional and proteomic levels. Also, we noted that the number of induced chaperones was higher atthe transcriptomic level than the proteomic one and increased as the fruit matured. Additionally, we identifiedclusters at both levels during advanced stages of ripening that were over-represented by HSP families (HSP70 andHSP20), chaperones (Proteasome assembly chaperone) and common protein interactors associated with proteinfolding, protein synthesis and degradation, and response to stress (Glutathione-S-transferase or GST; Endoplasmicreticulum auxin binding protein ER-ABP; tetratricopeptide repeat protein-ripening regulated protein orTRP-RRP). Finally, we found that some of these up-regulated chaperones show the presence of heat shockelement (HSE) motifs in their 5′ -untranslated regions (5´UTRs). Our combined inferential bioinformaticsapproach allowed us to integrate RNA, protein expression and co-expression levels of chaperones involved in cv.Micro-Tom fruit ripening.