Seasonal Variation of Plant Defense Inductor Ellagitannins in Strawberry Leaves under Field Conditions for Phytosanitary Technological Applications

CARLOS F. GRELLET; DI PETO, PÍA; CERVIÑO, A; MAMANÍ DE MARCHESE, A.; DIAZ RICCI, J.; FILIPPONE M.P,

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

AMER CHEMICAL SOC

Lugar: Washington; Año: 2021

0021-8561

Many natural compounds can activate the plant immunity, and for this reason, they have attracted special interest in8 crop disease management. Previously, we isolated from strawberry leaves an ellagitannin (HeT), which elicits plant defense9 responses. In this research, we investigated bioactive compounds from field-collected strawberry leaves capable of inducing defense10 responses in Arabidopsis thaliana against a bacterial pathogen. Methanolic extracts of strawberry leaves sampled at different months11 were obtained and compared. The highest content of total soluble phenolic compounds was found in the methanolic extracts of12 leaves sampled in December (DME). The defense response induced in A. thaliana by DME was attributed to two ellagitannins, the13 HeT and galloyl-HHDP-glucose. Both compounds exhibited phytoprotective effects against Pseudomonas viridiflava and induced the14 expression of PDF1.2 and PR1 genes. These results provide an economic value to strawberry leaves, normally discarded at the end of15 the harvest stage of the crop, as a raw material for plant health enhancer bioinputs.