Isolation and characterization of a subset of erythropoietin glycoforms with cytoprotective but minimal erythropoietic activity

MATTIO, MÓNICA; CEAGLIO, NATALIA; OGGERO EBERHARDT, MARCOS; PEROTTI, NORMA; AMADEO, GABRIEL IGNACIO; OROZCO, GUSTAVO; FORNO, ÁNGELA GUILLERMINA; KRATJE, RICARDO; ETCHEVERRIGARAY, MARINA

BIOTECHNOLOGY PROGRESS

AMER CHEMICAL SOC

Año: 2011 vol. 27 p. 1018 - 1028

8756-7938

Apart from its hematopoietic effect, erythropoietin(EPO) is a molecule with high neuroprotective potential.However, its prolonged application may cause seriousadverse effects due to the erythropoiesis stimulation.Therefore, an EPO derivative with neuroprotective propertiesbut low hematopoietic activity, designated as neuropoietin(rhNEPO), was developed in our lab using analternative purification process of the recombinanthuman erythropoietic counterpart (rhEPO) produced inCHO cells [1]. The in vitro cytoprotective activity ofrhNEPO on neural phenotype cells and its brain uptakefrom blood are herein analyzed.