Isolated soluble fractions from spleen cell culture supernatants induce tumor enhancement

A.M.EIJÁN,; M.A.JASNIS,; A.B.MOTTA,; S.OISGOLD-DAGÁ

JOURNAL OF SURGICAL ONCOLOGY

WILEY-LISS, DIV JOHN WILEY & SONS INC

Año: 1989 vol. 41 p. 134 - 138

0022-4790

J Surg Oncol. 1989 Jun;41(2):134-8. Isolated soluble fractions from spleen cell culture supernatants induce tumor enhancement. Eiján AM, Jasnis MA, Motta AB, Oisgold-Dagá S. Research Department, Institute of Oncology, A. H. Roffo, Buenois Aires,Argentina. Supernatants of spleen cell culture from small-tumor-bearing mice,large-tumor-bearing mice, and large-tumor resected mice were fractionated bySephadex G-100. The biological activity on tumor growth of all fractions wastested in vivo. It was found that only one fraction (MW: 220-250 kD) from spleen cell supernatants of small-tumor-bearing mice or large-tumor resected miceenhanced tumor growth. In spleen cell supernatants from large-tumor-bearing mice,two fractions had enhancing activity (MW: 220-250 and 100-10 kD). Thus, thesurgical resection of large tumor induced disappearance of enhancing activityfrom the fraction of lower molecular weight.  PMID: 2724981 [PubMed - indexed for MEDLINE]