Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability. Relationship with growth inhibition

G.BERTOLESI,; L.LAURIA,; E. S. DE LUSTIG,; A.M.EIJÁN

CANCER LETTERS

ELSEVIER IRELAND LTD

Año: 1995 vol. 90 p. 123 - 131

0304-3835

22. Cancer Lett. 1995 Apr 14;90(2):123-31. Heparin receptors in two murine mammary adenocarcinomas with different metastatic ability: relationship with growth inhibition. Esteban Bertolesi G, Lauría de Cidre L, Sacerdote de Lustig E, Eiján AM. Area Investigación, Instituto de Oncología A.H. Roffo, Universidad de BuenosAires, Argentina. Binding of heparin to primary cultured cells of two murine mammaryadenocarcinomas with low (M3) and high (MM3) lung, metastatic capacity wasdetermined. Heparin binding was rapid, specific and saturable. MM3 cells grownfor 24 h in fetal calf serum (FCS)-free medium exhibited a higher number ofbinding sites for 3H-heparin [(11 +/- 1) x 10(5) sites per cell than M3 cells[(6.9 +/- 0.6) x 10(5) sites per cell]. However, when M3 cells were grown in the presence of 2% FCS, they showed less heparin binding sites [(3.5 +/- 0.4) x 10(5)sites per cell]. In contrast, dissociation constants were very similar for MM3and M3 cells grown with or without FCS (Kd = 2-4 x 10(-9) M). Furthermore,heparin inhibited MM3 and M3 cell growth both in the absence or presence of FCS. Competition studies showed that chemically modified heparins lackingantiproliferative effect (O-desulfated; O/N-desulfated N-acetylated andN-desulfated heparins) were not able to inhibit 3H-heparin binding. N-desulfated N-acetylated heparin, which had partial antiproliferative effect, partiallyinhibited 3H-heparin binding, while heparin with a high antiproliferativeactivity inhibited more than 90% 3H-heparin binding. The antiproliferative effectof heparin and chemically modified heparins seems to be related to their binding ability to the cell membrane.  PMID: 7736447 [PubMed - indexed for MEDLINE]