INVESTIGADORES
MASSA Gabriela Alejandra
artículos
Título:
Evidence of Recombinant Isolates of Potato Virus Y (PVY) in Argentina
Autor/es:
COLAVITA MONICA LILIANA; MARCOS LANCIA; FEINGOLD SERGIO ENRIQUE; MASSA GABRIELA ALEJANDRA
Revista:
AMERICAN JOURNAL OF POTATO RESEARCH
Editorial:
SPRINGER
Referencias:
Lugar: Berlin; Año: 2017
ISSN:
1099-209X
Resumen:
In order to make a first approach in the identificationof the genetic diversity of Potato virus Y (PVY) inArgentina, 46 PVY isolates from different potato growingregions of Argentina were characterized both, biological andserologically. Five of them (ST11, RCA5a, RCA6, RCA14band SSF6) were selected for further genomic analyses. Fourgenomic fragments containing hot spot regions of recombination(HSR) reported previously were sequenced in each isolateand compared to PVYN (CS434575.1) and PVYO (U09509.1)reference genomes looking for genomic recombinations.Isolates with one, two or three recombination points wereidentified among these, including the two strains consideredtypical PVYN (RCA5b) and PVYO (SSF18) used as controls.This is the first report of the presence of recombined PVY inArgentina using a combination of biological, serological andmolecular tools that shed light on the genetic diversity of PVYviruses in this country.
