Study of the Mechanical and Structural Properties of Porcine-Derived Small Intestine Submucosa Decellularized with Triton X-100 at Different Concentrations

SORDELLI, ANDREA; JAUNARENA JH; LORESI M; DADAMO M; ALBITE R; COSTA L; LEONARDI, LUCIANO ; LONDOÑO CALDERÓN CL; FROIMOWICZ P; DEBADIOLA F; MOLDES JM; GIÚDICE C; GÜEGLIO G; VILLOLDO GM

Austin Journal of Biomedical Engineering

Austin Publications LLC

Lugar: Irving; Año: 2020

2381-9081

Introduction: Porcine-derived small intestine submucosa has been widelyused in tissue engineering. Cell components must be removed to precludetransplant rejection in animal models. Known as decellularization, this typeof process can damage collagenous fibers and thus affect the integrity of theextracellular matrix. Purpose: Determine whether structural and physical changesoccur in SIS when applying increased concentrations of a non-ionic detergent,Triton X-100, together with fixed concentrations of 0.1% sodium azide. Materialsand Methods: SIS was collected, sectioned and placed in distilled water for 24h before different concentrations of Triton X-100 were applied for 48 h. Sampleswere removed and stained with hematoxylin and eosin, Masson?s trichromeand Hoechst. Physical characterization was carried out via traction assays; wecalculated the rupture strain, ultimate tensile strength and Young?s modulus,and we performed infrared spectroscopy and thermogravimetric analyses. Wedetermined the concentration of remaining DNA.Results: Regardless of the concentration of detergent used, no cells wererevealed at 48 h post-treatment. The patterns of rupture strain, ultimate tensilestrength and Young?s modulus, as well as the infrared spectra, exhibited nosignificant differences between the use of high and low detergent concentrations.A higher detergent concentration results in a lower residual DNA concentration.Conclusions: It can be concluded that, irrespective of the concentrationof Triton X-100 used, at 48 hours post-treatment, decellularization is efficientand does not alter the structural or the physical characteristics of the material.Residual DNA amounts decrease with higher doses and longer treatment times.Keywords: SIS (Porcine-derived small intestine submucosa);Decellularization; Tissue engineering; Regenerative medicine; Characterization