Nanohydroxyapatite exerts cytotoxic effects and prevents cellular proliferation and migration in glioma cells

GOROJOD ROXANA MAYRA*; PORTE ALCON SOLEDAD*; DITTLER MARÍA LAURA; GONZALEZ MÓNICA CRISTINA; KOTLER MÓNICA LIDIA

TOXICOLOGICAL SCIENCES

OXFORD UNIV PRESS

Lugar: Oxford; Año: 2019 vol. 169 p. 34 - 42

1096-6080

Hydroxyapatite (Ca10(PO4)6(OH)2; HAP) is an essential component of the human bone inorganic phase. At the nanoscale level, nanoHAP (nHAP) presents marked emergent properties differing substantially from those of the bulk counterpart. Interestingly, these properties depend on nanoparticle characteristics. In this study, we investigated the cytotoxicity of rod-shaped crystalline nHAP (10- 20 nm x 50- 100 nm) in both normal (ARPE-19, BV-2) and tumoral (HepG2, HEp-2, A549 and C6) cells. We found that nHAP was cytotoxic in tumor HEp-2, A549 and C6 cells. Moreover, it induced an expansion of the lysosomal compartment at sub-lethal concentrations in different cell lines, while lysosomal membrane damage was not detected. In C6 glioma cells, the most sensitive cell line to nHAP, these nanoparticles increased reactive oxygen species (ROS) production and induced DNA damage measured by γ-H2AX phosphorylation. Interestingly, our data shows for the first time that nHAP affects two of the major pathways involved in cancer progression: cell unlimited proliferative capacity and cell migration. These results reveal novel antitumoral effects of nHAP in glioma and supportits possible usefulness for cancer treatment.