Strategies of detoxification and fermentation for biotechnological production of xylitol from sugarcane bagasse

VALLEJOS, M.E.; CHADE, M.; MERELES, E.B.; BENGOECHEA, D.I.; BRIZUELA, J.G.; FELISSIA, F.E.; AREA, M.C.

Industrial Crops and Products

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2016 vol. 91 p. 161 - 169

0926-6690

The aim of this study was to evaluate strategies ofdetoxification and fermentation of the hemicellulosic liquors obtained fromsugarcane bagasse autohydrolysis, for the biotechnological production ofxylitol. Different sequences of detoxification treatments were performed, andtheir effects on sugars loss and inhibitors removal were evaluated.Fermentation assays were accomplished with commercial xylose to select the bestyeast (C. guilliermondii, C. tropicalis) and the fermentation conditions of thedetoxified spent liquors. Detoxification through a sequence of treatments,including Ca(OH)2, IR-120 resin, activated charcoal, and IRA-67resin, practically removed all inhibitors from the hemicellulosic spentliquors. Maximal concentration of xylitol obtained was 32.0 g L−1 (C. tropicalis, xylose: 104.1 g L−1,yield: 0.46 g g−1, productivity: 0.27 g L−1 h−1).The technological parameters to obtain a detoxified spent liquor rich in xyloseand its bioconversion to xylitol were determined.