Kisspeptins and their receptors in the brain-pituitary-gonadal axis of Odonthestes bonariensis : Their relationship with gametogenesis along the reproductive cycle

TOVAR BOHÓRQUEZ, M. OSWALDO; MECHALY, ALEJANDRO S.; ELISIO, MARIANO; CHALDE, TOMÁS; CANOSA, LUIS F.; MIRANDA, LEANDRO A.; SOMOZA, GUSTAVO M.

GENERAL AND COMPARATIVE ENDOCRINOLOGY

ACADEMIC PRESS INC ELSEVIER SCIENCE

Año: 2017 vol. 252 p. 209 - 218

0016-6480

In vertebrates, the reproduction is controlled by the brain-pituitary-gonadal (BPG) axis and kisspeptinhas emerged as a key player of this axis. In this study, we analyzed changes in the expression levels ofkiss1, kiss2, and their receptors, kissr2 and kissr3 during gametogenesis in the BPG axis of feralOdontesthes bonariensis. In females, levels of brain kiss1 showed an increase at final maturation (Fm),while kiss2 levels were shown to be high at primary growth (Pg) stage, with no differences in the expressionof their receptors. In the pituitary, kiss1 and kiss2 peaked at the cortical alveoli (Ca) stage, and kissr3at initial vitellogenesis. In parallel, there was an increase of kiss1, kissr2 and kissr3 in the ovary during theCa stage and both receptors again at Fm stage. In males, the four genes were highly expressed in the brainat the arrested (A) stage. In the pituitary, kiss2 peaked at spermatogonial (SG) and spermatocytary (SC)stages; while kissr3 reached a peak at the spermiogenic stage (SP). In testes, kiss1 and kiss2 significantlyincreased during the SG and SC stages; meanwhile, kissr2 increased at SG and SC, whereas kissr3 levelswere significantly high at SC and SP stages. Taken together these results showed that the kisspeptin systemin pejerrey is expressed in the three levels of the BPG axis with different expression profiles duringthe gonadal cycle. These findings pointed that kisspeptins have different roles in gametogenesis in thisspecies.