INVESTIGADORES
ESTEVEZ Jose Manuel
artículos
Título:
Tetracystein-FIAsH-based approach to visualized synthetic hydroxyproline rich-olipeptide with AGP-motif expressed in Arabidopsis and Tobacco.
Autor/es:
ESTEVEZ, J. M. & C. SOMERVILLE
Revista:
BIOTECHNIQUES
Referencias:
Año: 2006 vol. 49 p. 569 - 572
ISSN:
0736-6205
Resumen:
Genes encoding synthetic hydroxyproline-rich peptides with repetitive (Ser-Pro) units linked to an extensin signal sequence and a tetra-cysteine sequence were expressed transiently in tobacco and transiently and stably in Arabidopsis under control of a strong constitutive promoter. Expression of these peptides could be visualized in live cells by confocal microscopy following labeling with FlAsH- or ReAsH-EDT2 reagents.
