DMSO supplementation during in vitro maturation of bovine oocytes improves blastocyst rate and quality

YNSAURRALDE, AE.; SUVA, M.; LUCHETTI, CG.; BEVACQUA, RJ.; MUNILLA, S.; RODRIGUEZ-ALVAREZ, LL.; VELASQUEZ, A.; BRISKI, O.; LOMBARDO, DM.; SALAMONE, D.

THERIOGENOLOGY

ELSEVIER SCIENCE INC

Lugar: Amsterdam; Año: 2020 vol. 148 p. 140 - 148

0093-691X

Dimethyl sulfoxide is a molecule widely used as drug solvent. However, its antioxidant property is poorly explored. In this study we evaluated the effect of DMSO supplementation during oocyte in vitro maturation on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0.1-10%) followed by in vitro fertilization. Subsequently, quality indicators such as oocyte and embryo redox status, gene expression of SOX2, OCT4, CDX2 Y SOD1 and DNA damage was evaluated. 10% of DMSO did not produce blastocyst whereas concentrations up to 1% did not increase first polar body extrusion and cleavage rates. Moreover, 0.25 and 0.5 % v/v DMSO increased blastocyst rates. The optimal concentration of DMSO to use in the maturation was estimated in around 0.45% v/v. Concentrations higher than that increased gene expression of OCT4 and SOX2. The results of redox balance demonstrated that reduces lipid peroxidation and increases GSH in oocytes and blastocysts. Moreover, DMSO increased the total cell number of blastocysts but not the TUNEL positive cells. In conclusion, DMSO used during in vitro maturation of bovine oocytes has a clear effect on the machinery that regulates oxidative stress and, consequently, increases the blastocyst rate and quality.