Location of high affinity neurotrophins receptors in rabbit´s (Oryctolagus cuniculus) thymus

GAUNA AÑASCO, LG; SOÑEZ, MC.; DI MATTEO, AM; GAZZANEO, P.; LOMBARDO, DM.; VEGA ALVAREZ, JA

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza. Argentina; Año: 2009 vol. 33 p. 191 - 223

0327-9545

Neurotrophins are growth factors involved in the development of central and peripheral nervous system and lymphatic system of domestic and wild animals that we have studied. The objectives of this work were to determine the localization of high affinity receptors in rabbit’s thymus and compare with others domestic and wild species (pigeon, chicken, pig, bovine, horse, llama, caiman, fish, armadillo and human). Samples from 12 young rabbit thymus were fixed in 10% buffered formaldehyde and processed by the routine histological technique, included in paraffin. On serial sections an indirect immunocytochemistry method (ABC) was applied employing anti- Trks A, B and C diluted 1:100. Negative controls consisted in the omission of the first incubation with the primary antibody. TrkA was expressed in reticulo-epithelial cells type III and IV similar to those that were localized in pigeon, human and horse, and cortex stroma. TrkB was expressed in monocyte-macrophagic cells of cortex and medullar area like fish, pigeon, mouse and llama species. TrkC was identified in dendritic cells around Hassall corpuscles in medullar area, similar results were observed in rat and pig. Trks proteins may play a paracrine function for an adequate environment for T lymphocytes maturation in rabbit’s thymus.