Expresión of high affinity Neurotrophins Receptor (Trks Proteins) in Sus scrofa domesticus Secondary Lynphatic Organs

GAUNA AÑASCO, LG; DI MATTEO, AM; SOÑEZ, MC; LOMBARDO, DM; VON LAWZEWITSCH, I.; VEGA, JA

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza. Argentina; Año: 2007 vol. 31 p. 344 - 354

0327-9545

EXPRESSION OF HIGH AFFINITY NEUROTROPHINSRECEPTORS (Trks PROTEINS) IN Sus scrofa domesticusSECONDARY LYMPHATIC ORGANSGauna Añasco LG1, Di Matteo AM1, Soñez MC1, Lombardo DM1,Von Lawzewitsch I1, Vega JA2.1Fac. de Cs. Veterinarias. Histología y Embriología. UBA, Argentina.2Universidad de San Pablo. Madrid. España. E-mail:gauna@fvet.uba.arThere are evidences that neurotrophins can work on other tissuesthat express mRNA and/or their receptors, as rats, mice and manlymphatic system. This investigation goal was to determine Trkreceptors localization in pig’s secondary lymphatic organs (spleen,lymphnodules, tonsils and Peyer´s patches). Samples from youngpigs (n=20) were fixed in 10% buffered formaldehyde and processedby routine histological techniques. Trks proteins were labeledwith rabbit polyclonal antibodies diluted 1:100 (Santa CruzBiotechnology, CA, USA), employing the immunocytochemistryavidine-biotine-peroxidase method (ABC).Spleen: TrkA and TrkB were localized in white pulp and in bloodvessels inside lymphoid follicles. Trk C was identified in capsule,trabecules, and lymphoid tissue of red pulp.Lymphnodules follicles: TrkC was found in cortical lymphoid tissueand capsule. TrkA and TrkB had negative immunoreactivity.Palatine tonsils: TrkA and TrkC were identified in stroma follicleswhile TrkB was negative.Peyer‘s patches: TrkA and TrkC were shown in lymph folliclescenter, TrkB showed negative immunoreactivity.We conclude that TrkA and TrkC studied expression in pig’s secondarylymphatic organs would indicate the probably action of NGFand NT-3 growing factors, while TrkB, was identified only in spleen,this would suggest a possible role of BDNF and NT-4/5 as growingfactors in this organ, for maintenance of cellular populations.