Changes in Ovaries of Spotted Tinamous (Nothura maculosa Tinamidae, Temminck, 1815) related to gonadotrope population.

CLAVER J; ROSA JM; LOMBARDO DM; SOÑEZ MC

INTERNATIONAL JOURNAL OF MORPHOLOGY

Universidad Nacional de La Frontera

Lugar: Ciudad de Temuco. Chile; Año: 2008 vol. 26 p. 353 - 361

0717-9367

SUMMARY: Nothura maculosa is a South American Tinamidae with a marked seasonal reproductive pattern. This work describes ovarian seasonal changes in this species related to gonadotrope (GTHs) population. Ovary and pituitary samples were collected monthly from adult birds during four annual periods. They were fixed in Bouin’s solution and processed for light microscopy. The data of post-fixation gonadal weight were analysed using STATISTIX 4.0. Histological sections of the ovaries were stained with H/E, PAS and Goldner-Masson trichrome. Single and double immunostaining were applied on pituitary sections with anti-chicken-FSH and anti-chicken-LH antibodies. The samples were analysed in quarterly periods of the year, P1: March-May (resting stage); P2: June-August (developing stage); P3: September-November (reproductive stage); P4: December-February (involutive stage). Ovary weight (ow) significantly varied among periods (p