Apoptotic effect of an analog of GnRH (acetate of Leuprolide ) on an established line of Bovine Granulosa cells

CAROU, MC.; LOMBARDO DM.M; SOÑEZ MC.C; MEDINA, JM.

BIOCELL

INST HISTOL EMBRIOL-CONICET

Lugar: Mendoza. Argentina; Año: 2006 vol. 30 p. 505 - 505

0327-9545

&amp;lt;!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman";} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page WordSection1 {size:595.3pt 841.9pt; margin:70.85pt 271.3pt 449.25pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.WordSection1 {page:WordSection1;} --&amp;gt; Apoptotic effect of an analog of GnRH (acetate of Leuprolide ) on an established line of Bovine Granulosa cells. Carou MC., Lombardo DM., Soñez MC. and Medina J. Faculty of Veterinary Science. UBA. E-Mail: mccarou@fvet.uba.ar   It is known that the GnRH is also expressed in the ovarian tissue. GnRH receptors (GnRHr) in the granulosa (GC) and luteal cells have been identified. Analysis of the hormonal regulation with agonists of GnRH (GnRH-a) in Vivo and in Vitro, have demonstrated an increase of the atretic follicles in ovaries of rats. Also, have been determined that the in Vivo administration of GnRH-a increases the cellular death by apoptosis in ovarian tissue and GC. The objective of work is to analyze in Vitro if a GnRH-a, the acetate of Leuprolide (LA), has apoptotic effect on an established line of bovine granulosa cells (BGC). For it, three experiment of activation were made (6, 12 and 24 hs) and three doses of LA (1, 10 and 100 nM), under two experimental conditions (0 and 5% of bovine fetal serum). Each experiment was made by triplicate analyzing the results by ANOVA two way and a Bonferroni´s test comparison averages. The apoptosis index was determined by the DAPI chromatin stain, validating the results by TUNNEL. Analyses and comparison of the results show activation of the apoptosis and significant differences respect to control (44% activation, p <0,001) at the 24 hs of incubation in the presence of 5% SFB and LA 100 nM. This result it was validated by TUNNEL, being in the same conditions a 39% of activation. Similar results were obtained in bovine GC of primary culture of follicles of medium size (5 to 8 mm). Finally we concluded that LA active the apoptosis processes in the system of BGC and for these experimental conditions.