Bovine herpesvirus type 5 replication and induction of apoptosis in vitro and in the trigeminal ganglion of experimentally-infected cattle

DANIEL E. RENSETTI; MAIA S. MARIN; PEDRO E. MORÁN; ANSELMO C. ODEÓN; ANDREA E. VERNA; SANDRA E. PÉREZ

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2018

0147-9571

Bovine herpesvirus (BoHV) types 1 and 5 are neuroinvasive. Cases of BoHV-1-induced encephalitis are not asfrequent as those caused by BoHV-5. In this study, the capability of BoHV-5 to induce apoptosis in cell culturesand in the trigeminal ganglion during acute infection of experimentally-infected cattle was analyzed. Apoptoticchanges in cell cultures agree with the ability of the viral strains to replicate in each cell line. Marked differenceswere observed between the in vitro induction of apoptosis by BoHV-1Cooper and BoHV-5 97/613 strains.Apoptotic neurons were clearly evident in the trigeminal ganglion of BoHV-1-infected calves. For BoHV-5 afewer number of positive neurons was observed. There is an association between the magnitude of bovineherpesviruses replication and the induction of apoptosis in trigeminal ganglion. These findings suggest that theinduction of apoptosis and the innate immune response orchestrate the final outcome of alpha herpesvirusesinfection of the bovine nervous system.