INVESTIGADORES
MARTINEZ Oscar Eduardo
artículos
Título:
Single Photon Fluorescent Microlithography for Live-Cell Imaging
Autor/es:
DARÍO KUNIK; PEDRO F. ARAMENDÍA; OSCAR EDUARDO MARTÍNEZ
Revista:
MICROSCOPY RESEARCH AND TECHNIQUE.
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Lugar: New York; Año: 2010 vol. 73 p. 20 - 26
ISSN:
1059-910X
Resumen:
rapid fabrication of micrometer and submicrometer sized fluorescent structures by
one-photon absorption. Here, we show that standard HeNe lasers emitting at 632.8 nm can be
used to start the photopolymerization and that very low laser power is required. This procedure
allows the fabrication of fiduciary fluorescent references on standard glass coverslips, mica sheets,
or gold-coated coverslips for laser scanning or standard fluorescent microscopy. The biocompatibility
of the polymerized resin with cells in culture was tested by growing Xenopus melanophores and
a standard laser scanning microscope was used to demonstrate that it is possible to use equipment
readily available in several laboratories. We show that fluorescent structure with less than 10 nm
in height may be used as references in fluorescence microscopy allowing a smooth environment for
cell growth. Different dyes were tested and the conditions for one-photon polymerization were
outlined.