INVESTIGADORES
MARTINEZ Oscar Eduardo
artículos
Título:
Distance and orientation measurement in the
Autor/es:
HERNÁN E. GRECCO; OSCAR E.MARTINEZ
Revista:
OPTICS EXPRESS
Editorial:
OSA
Referencias:
Año: 2006 vol. 14 p. 8716 - 8721
ISSN:
1094-4087
Resumen:
Abstract: We show that the orientation of a dimer and the distance between
the nanoparticles that form it can be determined by measuring the scattering
under polarized light illumination. Scattering microscopy has shown to be
an alternative to fluorescence as it provides nonbleaching and highly
biocompatible probes, that can be manufactured in different sizes with
different ligands. We propose a method based on measuring the polarization
anisotropy of metallic dimers to determine distances in the range from 10
nm to 200 nm, thus filling the gap between fluorescence resonance energy
transfer (FRET) and conventional microscopy. By calculating the scattering
cross section of metallic dimers we show that it is also possible to gather
orientation information, relevant to understand many biological processes