INVESTIGADORES
COMELLI Raul Nicolas
artículos
Título:
Transcriptional coordination of the biogenesis of the oxidative phosphorylation machinery in plants
Autor/es:
GONZALEZ, DH; WELCHEN, E; ATTALLAH, CV; COMELLI, RN; MUFARREGE, EF
Revista:
PLANT JOURNAL
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Año: 2007 vol. 51 p. 105 - 116
ISSN:
0960-7412
Resumen:
Publicly available microarray experiments
were used to analyze Arabidopsis thaliana genes whose expression is
correlated with that of nuclear genes encoding components of the
oxidative phosphorylation machinery (OxPhos genes). This analysis
indicated the existence of coordination in the expression of genes
encoding components of the five respiratory complexes. For these genes,
preferential expression was observed in anthers and roots, especially in
the elongation zone, while reduced or very low relative expression was
evident in leaves and mature pollen grains. A global induction of OxPhos
genes by carbohydrates, photo-destruction of chloroplasts, inhibition
of cellulose synthesis, release from dormancy and germination, among
other conditions, was also observed. Cluster analysis of the response of
Arabidopsis genes to a set of 15 treatments allowed the identification
of DNA motifs, known as site II, that are frequently present in the
upstream regions of genes with responses like those of OxPhos genes.
Mutagenic analysis of site II motifs in several genes encoding
respiratory chain components showed that they actively participate in
transcription of these genes. We conclude that an important number of
nuclear genes encoding components of the five respiratory complexes show
coordinated expression under various circumstances, and that site II
elements and the putative proteins that interact with them are, together
with as yet unidentified factors, important actors in this coordinated
response.